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Welcome to the Cerebellar Gene Regulation in Space & Time Database

Overview


Microarray

The Cb GRiTS Database resource represents a growing number of datasets for exploring cerebellar development of the mouse in time and space.

The database contains a number of mutant/wildtype microarray datasets including two complete wildtype microarray time-series (C57BL/6 and DBA/2J).  Our time-series data spans twelve time-points between E12-P9.  The dataset also includes in situ hybridization and bioinformatic analyses.


In Situ Hybridization

Exploration of this dataset will allow the investigator to assess differential gene expression profiles from a developing mutant cerebella, to assess the temporal changes in gene expression in the wildtype, and to verify the cellular expression of these genes in images from our in situ hybridization library.


Bioinformatic Analysis

Using our database, the investigator can explore the developmental expression or differential expression patterns of a particular gene, or create lists of similarly expression genes by building simple search algorithms.  These lists can then be mined accross all the datasets in both space and time.

Current Datasets

Our current datasets represent gene expression analyses from multiple cerebellar mutant and wildtype single time-point and developmental series.

  • C57BL/6 WT Time Series - A developmental time-series (E12, E13, E14, E15, E16, E17, E18, E19, P0, P3, P6, P9) from wildtype C57BL/6 mice.  Run on Illumina v1.1 platforms. [Goldowitz Lab, CMMT]
  • DBA/2J WT Time Series - A developmental time-series (E12, E13, E14, E15, E16, E17, E18, P0, P3, P6, P9) from wildtype DBA/2J mice.  Run on Illumina v1.1 platforms. [Goldowitz Lab, CMMT]
  • Math1 Mutant (E15.5) - A single time-point (E15.5) from mutant and wildtype littermates carrying the Rhombic lip derivative gene, Math1. Run on an Affymetrix U74a_v2 and M430A&B platforms. [Goldowitz Lab, UTHSC]
  • Meandertail Mutant - A single time-point (E15.5) from mutant and wildtype littermates carrying the presumed granule cell specific, "Meandertail," gene mutation. Run on an Affymetrix M430v2.0 platform. [Goldowitz Lab, UTHSC]
  • Pax6 Sey Mutant Time Series - A developmental time-series (E13.5, 15.5, 18.5) from mutant and wildtype littermates carrying the cerebellar granule cell specific gene, Pax6, gene null mutation. Run on an Affymetrix U74a_v2 platform. [Goldowitz Lab, UTHSC]
  • Riken Mut/WT Time Series - A developmental time-series (E18.5, P7, P14, P21, P56) from wildtype C57BL/6 mice. Run on the Affymetrix MU11K platform. [Teiichi Furuichi, Riken Institute]
  • Staggerer Mut/WT Time Series - A development time series (E15.5, E17.5, P0, P1, P4) from mutant and wildtype littermates carrying the Purkinje cell specific, RORa, knockout mutation. Run on an Affymetrix U74a_v2 platform. [Bruce Hamilton, UCSD]
  • Zic 1/4 Mut/WT Time Series

Future additions to the database will include a developmental series from BXD RI lines and an extensive survey of additional cerebellar mutant lines.

Project funded by funded by NIH Grant HD052472. Please view our funding & data sharing policy
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